Accumulation of Phosphorylated Repressor for Gibberellin Signaling in an F-box Mutant Akie Sasaki,1* Hironori Itoh,1* Kenji Gomi,1 Miyako Ueguchi-Tanaka,1 Kanako Ishiyama,2 Masatomo Kobayashi,2 Dong-Hoon Jeong,3 Gynheung An,3 Hidemi Kitano,4 Motoyuki Ashikari,1 Makoto Matsuoka1

Gibberellin (GA) regulates growth and development in plants. We isolated and characterized a rice GA-insensitive dwarf mutant, gid2. The GID2 gene encodes a putative F-box protein, which interacted with the rice Skp1 homolog in a yeast two-hybrid assay. In gid2, a repressor for GA signaling, SLR1, was highly accumulated in a phosphorylated form and GA increased its concentration, whereas SLR1 was rapidly degraded by GA through ubiquitination in the wild type. We conclude that GID2 is a positive regulator of GA signaling and that regulated degradation of SLR1 is initiated through GA-dependent phosphorylation and finalized by an SCFGID2-proteasome pathway.

1 BioScience Center, Nagoya University, Nagoya 464-8601, Japan. 2 BioResources Center, Riken, Tsukuba 305-0074, Japan. 3 Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea. 4 Graduate School of Bioagricultural Science, Nagoya University, Nagoya 464-8601, Japan. * These authors contributed equally to this work.

To whom correspondence should be addressed. E-mail: makoto@agr.nagoya-u.ac.jp

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